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MTT Cell Proliferation Assay 1 MTT salt : prepare a stock solution of MTT fresh each time just prior to use Add

Moreover, since the MTT assay relies on the reduction of MTT to Prepare the MTT stock solution at a concentration of mgml in PBS

mtt stock Note: Incubation time will vary depending on cell line and cell density Prepare MTT stock solution 2 Dissolve 5 mg MTT in 1 ml 1X PBS

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