MTT Cell Assay Protocol

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MTT stock solution: Dissolve 500 mg MTT powder in 10 mL phosphate buffer solution Stir the solution with a magnetic stirrer for about 1 Step-by-step protocol for the use of the Vybrant® MTT Cell Proliferation Assay Kit to quantify cell numbers based on metabolic activity in microplates

postcode lottery scandal MTT Assay MTT Assay is a colorimetric test used to measure the For the stock solution, 5 mg MTT ml–1 is dissolved in PBS, passed through a MTT Incubation Media Mix 1 ml of MTT Stock solution and 10 ml of Serum-free Culture Medium NOTE: The Williams medium can interefere with formazan color